Publications

My research contributions to the field of bioinformatics and computational biology, featuring peer-reviewed articles and manuscripts under review.

2 Peer-Reviewed Articles
2 Manuscripts Under Review
1 Conference Presentation

Peer-Reviewed Publications

2024

Development of an In Silico Platform (TRIPinRNA) for the Identification of Novel RNA Intramolecular Triple Helices and Their Validation Using Biophysical Techniques

Isha Rakheja*, Vishal Bharti*, Souvik Maiti, Debojyoti Chakraborty
* Equal contribution
Biochemistry 2024 DOI: 10.1021/acs.biochem.4c00334

Abstract

We present TRIPinRNA, a computational platform designed to predict intramolecular RNA triple helix structures with high accuracy. Using a combination of thermodynamic calculations and structural constraints, our algorithm identifies potential triple helix forming regions in RNA sequences. We validated our predictions using biophysical techniques including circular dichroism spectroscopy and thermal denaturation studies. Our analysis reveals that triple helices are more prevalent in the human transcriptome than previously recognized, with significant enrichment in long non-coding RNAs involved in gene regulation and chromatin organization.

2024

IGF2BP1-Mediated Regulation of CCN1 Expression by Specific Binding to G-Quadruplex Structure in its 3´UTR

Pallabi Rana, Utkarsh Rajat, Vishal Bharti, Tanmoy Mondal, Bincy P. Baby, Pratik Chakraborty, Souvik Maiti, Debojyoti Chakraborty
Biochemistry 2024 DOI: 10.1021/acs.biochem.4c00172

Abstract

This study elucidates how G-quadruplex structures in the 3'UTR of CCN1 mRNA regulate gene expression through specific binding by IGF2BP1. Using a combination of computational predictions, biophysical characterization, and proteomics analysis, we demonstrate that IGF2BP1 recognizes and stabilizes G-quadruplex structures, leading to enhanced CCN1 expression. Our findings reveal a novel mechanism of post-transcriptional regulation where RNA secondary structures serve as binding platforms for regulatory proteins, with implications for understanding cellular proliferation and wound healing processes.

Manuscripts Under Review

2023

An Interface of Genetically Engineered Human Forebrain Assembloids and Polymeric Nanofiber Scaffolds for Multiscale Profiling of Interneuron Migration Disorders

Rahul Rauthan, Vishal Bharti, Aditya Mittal, Suparna Mukherjee, Sumeet K. Asthana, Divya Tej Sowpati, Dipyaman Ganguly, Debojyoti Chakraborty, Apratim Bajpai, Sudipta Maiti, Shubha Tole, Sanjay Singh
Under revision at Stem Cell Reports 2023 Preprint: 10.21203/rs.3.rs-3831019/v1

Abstract

We present a novel platform combining human forebrain organoids with polymeric nanofiber scaffolds to study interneuron migration disorders. Through comprehensive computational analyses including RNA-seq, protein-protein interaction networks, and pan-tissue expression analysis, we identified key molecular pathways disrupted in neurodevelopmental disorders. Our multiscale approach reveals how physical scaffolds can rescue migration defects, providing insights into potential therapeutic strategies for conditions like autism spectrum disorder and epilepsy.

2023

MLC1 alteration in iPSCs give rise to disease-like cellular vacuolation phenotype in the astrocyte lineage

Shyamala Sharma, Vishal Bharti, Prasanna Kumar Das, Nikhil Masurkar, Chintan Joshi, Gargi Kulkarni, Bharat Gogisetty, Snehal Dhamale, Sudhakar Sivasubbu, Apratim Bajpai, Debojyoti Chakraborty, Sourav Banerjee
bioRxiv 2023 DOI: 10.1101/2025.01.06.631607

Abstract

This study investigates the cellular and molecular mechanisms underlying Megalencephalic Leukoencephalopathy with subcortical Cysts (MLC) using patient-derived iPSCs. I led the RNA-seq data analysis and developed a diagnostic platform to identify the 132dupC MLC1 gene mutation. Our findings reveal how MLC1 mutations lead to astrocyte dysfunction and characteristic vacuolation phenotypes, providing insights into disease pathogenesis and potential therapeutic targets for this rare neurological disorder.

Conference Presentations

2023

Boundary Without Fence

Vishal Bharti, Debojyoti Chakraborty
Poster Presentation International Conference: Frontiers in Genome Engineering 2023 BITS Pilani Goa Campus

Abstract

This poster presented our work on understanding how chromatin boundaries are established and maintained without physical barriers. Using computational approaches to analyze CTCF binding sites and chromatin accessibility data, we demonstrated how dynamic interactions between architectural proteins create functional boundaries in the genome. The work has implications for understanding gene regulation and chromatin organization in health and disease.

Research Impact & Metrics

Citations

Growing Impact

Recent publications gaining recognition in the scientific community

Collaborations

30+ Co-authors

Working with researchers across multiple institutions

Research Areas

4 Major Domains

RNA Biology, Genomics, Neuroscience, Bioinformatics Tools

Open Science

100% Open Access

All publications freely available to the research community

Collaboration & Inquiries

I'm always interested in discussing research collaborations, sharing data and code, or answering questions about my publications. Feel free to reach out!